BMC Biotechnol ;Dec This study highlights the presence of stem cells in DF, their potential to adhere and differentiate into osteoblasts on different types of titanium surfaces.
The presence of growth factors until 28 days in medium maintained the cells in an earlier stage of differentiation with a lower level of specific bone proteins and a higher expression of alkaline phosphatase ALP.
Influence of titanium implants with different bioactive coatings, hydroxyapatite TiHA and with silicatitanate TiSiO2and porous Ti6Al7Nb implants as control TiCtrlwas studied in terms of cell adhesion and breast cancer genetic or environmental.
Ti HA implants proved to be more favorable for adhesion and proliferation of DF stem cells in first days of cultivation.
The influence of titanium coatings and osteogenic differentiation mediums with or without growth factors were evaluated. Additional BMP-2 in the medium did not allow DF stem cells to develop a more mature phenotype, leaving them in a pre-osteogenic stage.
Long term culturing 70 days breast cancer genetic or environmental titanium surfaces of DF stem cells in standard medium without soluble osteogenic inducers, indicated that HA coating is more favorable, with breast cancer genetic or environmental acquisition of a more mature osteoblastic phenotype as shown by immunocytochemical staining.
These findings demonstrated that even in absence of exogenous osteogenic factors, TiHA implants and in a lesser extent TiCtrl and TiSiO2 implants can induce and sustain osteogenic differentiation of DF stem cells, by their chemical and topographical properties.