In case of dead animals, the specimens shluld be taken soon after seath to avoid the chances of invasion of tissues by putrefactive bacteria rendering the specimens unsuitable for examination. Generous blocks of fresh tissues such as liver, spleen, kidneys, lymph nodes, lungs and brain may be forwarded refrigerated but not frozen in wide mouth sterile bottles, when the examination is to be carried out within a short period after collection.
While forwarding material in this preservative, it would be better to send larger pieces of the tissues, so that if helminth disease burden, cultures may be attempted from their central areas where the glycerine penetration has been the least. Liquid helminth disease burden such as heart blood, cerebrospinal fluid and inflammatory exudates may be taken either on sterile swabs, sealed in pipettes or collected in tubes or bottles with strict aseptic precautions.
The surface of the organ should be well seared with a hot spatula and a sterile syringe and needle or pipette inserted into it for drawing the material.
Trichuris Trichiura, or Whipworm
If the peritoneal fluid is required, an area on the abdominal wall is helminth disease burden thoroughly and a sterile knife is used for holding cancer peritoneal dolor cavity open and sterile pipettes for drawing the fluid.
For taking swabs of the contents of a closed abscess in a living animal, clip the hair from the area and paint it with tincture of iodine. Open the abscess with a sterile scalpel and take swabs from the wall as well as from the contents of the abscess and keep the swabs in sterile tubes.
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To collect wound discharge, the wound should be thoroughly cleaned with warm water and soap, and sterile non-antiseptic cotton wool or gauze dressing applied. The material should be collected after about 24 hours by inserting a sterile swab underneath the dressing.
For bacteriological examination of the intestinal flora, ligate about 6 inches of the bowel at both ends and forward the loop unopened under refrigeration. If there are abnormal contents in the uterus, a small segment is isolated between ligatures and retained unopened for bacteriological examination.
While it is desirable that the specimen for immunological study be refrigerated in transportation, serum for agglutination and complement fixation test may be preserved with 0.
In swine, organisms are not present in blood, so swabs should be taken from exudates and the cut surface of hemorrhagic lymph nodes.
Flame fixed blood helminth disease burden disease burden of cattle and sheep. From subcutaneous swelling in horses, swine and dogs.
Swabs of blood from ear vein for cultural examination from dead animals. It is not advisable to open the carcass suspected for anthrax in the field. If opened, it should be properly disposed by burning.
Blackleg Black Quater : Impression smears from the affected muscle tissue; exudate from lesions; pieces of affected muscles on ice. Tetanus: Material from wound site isolation is not usually attempted Bacillary Haemoglobinuria: Affected liver tissue.
Botulism: Suspected food, meat, forage and urine. Enterotoxaemia, Lamb Dysentery: Intestinal pieces with contents inside tied with thread or contents of small intestine with and without chloroform helminth disease burden on ice, kidney, urine. Campylobacteriosis: Prepeutial washings, semen, foetal stomach contents, cervicovaginal mucus helminth disease burden should reach the laboratory within 6 hours of collection under refrigeration or at room temperature in transport media.
Brucellosis: Paired sera samples, blood and abomasal contents of aborted foetus, placenta with cotyledons, vaginal swabs in PBS, separate bottle on ice, whole foetus if small on ice.
For ABR milk is used avoiding colustrum and milk from drying off animals or those suffering from mastitis. Milk, serum, vaginal mucous etc.
Haemorrhagic Septicemia: From sick animals fixed smears from blood and throat swelling and from dead animals, smears from heart blood and liver. Glanders: Exudate from skin and ung lesions in vials on ice. Impression smears from exudate duly fixed, tissue containing early nodules, pus from ulcers.
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Salmonella Sp. Paired sera samples. After collection, ectoparasites and intermediate hosts can be sent as such or after fixation. The chloroform is allowed to settle and the top solution is poured constituting the chloroform formalin mixture which retains the natural colour of ticks, if dropped alive. Fleas and lice are collected on a sheet of paper either by combing or with the help of a camel hair brush moistened with xylene.
Dead small birds and animals can be transported as such in securely tied polythene bags.