Rotar, Florin V. Interleukin 1β is a proinflammatory cytokine that plays a critical role in chronic inflammation and carcinogenesis.
Material and method.
A case-control study was realized. The cases were represented by patients diagnosed with cervical intraepithelial neoplasia, positive at HPV Cervical cancer bethesda classification testing, while controls, negative for intraepithelial neoplasia and negative at HPV HR testing, were included in the control group.
Chi-square statistics had cervical cancer bethesda classification value of 0. Odds ration had values of 1. Designul studiului a fost de tip cervical cancer bethesda classification.
Lotul de studiu a fost reprezentat de de paciente diagnosticate cu neoplazie cervicală intraepitelială şi pozitive la testarea HPV, iar lotul de control a fost reprezentat de paciente fără leziuni cervicale şi negative la testarea HPV.
Cervical cytology - The Bethesda System, Normal Pap (Squamous component).
The mechanisms involved in this transition are extremely complex and only partially understood, immune signal molecules, chemokines, interleukins and the molecules involved in the control of the cellular cycle being involved 1.
Genetic particularities of these molecules could explain why fortunately only a small number of HPV Human Papilloma Virus infected women will develop cervical cancer.
Interleukin 1b together with interleukin-1a and the antagonist of interleukin 1 belong to the interleukin 1 cytokine family, located on a kb segment on chromosome 2. Interleukin 1b is synthesized mainly by macrophages, but also by other cells of the immune system, with important roles in both acute cervical cancer bethesda classification chronic inflammatory response 2.
The fact that chronic HPV infection is a mandatory step in cervical carcinogenesis is well known 3.
Genetic variants of Interleukin 1-Beta-511 C>T and cervical intraepithelial neoplasia
Previously published researches confirmed the contribution of interleukin 1b in the neoplastic process by influencing tumor growth and angiogenesis 4respectively in the induction of chemo resistance 5. While performing a search throughout MEDLINE databases we have encountered articles regarding the association between this polymorphism and human pathologies 8.
Он, разумеется, может возвратиться, как только сам этого захочет или же как только понадобится .
- Corelaţii clinice şi paraclinice în managementul neoplaziei intraepiteliale cervicale
- Variantele genice ale Interleukinei 1β C>T şi neoplazia intraepitelială cervicală
- Cancer de piele pe picior
The presence of this polymorphism was found to be significantly associated with gastroduodenal ulcer 9neoplasia 10pulmonary 11 and pancreatic cancer This particular genetic cervical cancer bethesda classification was proved to be related to gynecological and obstetrical conditions: high risk of preterm labor 16spontaneous recurrent abortions 17,18bacterial vaginosis 19polycystic ovary syndrome 20 and uterine leiomyoma The relationship between this genetic polymorphism and cervical cancer was previously analyzed in two small studies in an Indian and respectively Korean population but not in Caucasians 22, One hundred and eleven patients with negatives results for cervical cancer bethesda classification lesion with negative HPV HR tests were included in the control group.
The patients were recruited from the corpus of patients hospitalized or only consulted on an ambulatory base during this period at the First Clinic of Obstetrics and Gynecology, Emergency County Hospital Cluj-Napoca, Romania, willing to participate at this study.
Variantele genice ale Interleukinei 1β -511 C>T şi neoplazia intraepitelială cervicală
The study was approved by the ethical committees and the written consent was obtained for each patient Biological probes collection A gynecological examination was performed for each patient. A cervical cytology was obtained. Cervical cytology results were formulated according to Bethesda nomenclature Colposcopic examination was performed for each patient. After the gynecological examination a blood sample was obtained using a 2 ml blood collection tube.
The blood samples were immediately stored at °C. The analysis of cervical samples The cervical cytology was examined by a cytologist after a preliminary Papanicolau staining in the laboratory of the above mentioned hospital.
HPV HR testing was performed using an immunohistochemistry-based kit. DNA extraction. Genetic analysis The genomic DNA was extracted from peripheral leukocytes contained in µl of whole blood using a commercial kit Wizzard Genomic DNA Purification Kit, Promega® according to the producer instructions.
- This is achieved by the excision or ablation of the squamous-cylindrical area up to the healthy tissue.
- Detoxifiere cu clean 9
- Cervical precancer | Cabinet ginecologie
The extracted DNA was stored in a freezer at °C. Briefly, the PCR reaction was done cervical cancer bethesda classification 25 µl, total volume containing: Free nuclease water was added in order to reach the total volume.
Clinical and paraclinical correlations in the management of cervical intraepithelial neoplasia
The PCR protocol is shown in Table 2. Table 1. Statistical analysis The quantitative continuous variables were expressed using centrality and dispersion parameters.
Comparison of the distribution of genotypes on cases and controls was done using chi-square test.
The differences were considered significant if p-value was lower than 0. CatROM program 28 was used in order to calculate the statistical parameters associated to the 2×2 contingency table for investigation of genetic polymorphisms as risk factor for cervical cancer. Results One hundred and twenty eight cases and one hundred and eleven controls were enrolled in this study.
Each case had a diagnosis of cervical intraepithelial neoplasia based on cervical cytology.
Corelaţii clinice şi paraclinice în managementul neoplaziei intraepiteliale cervicale
In patients with intraepithelial neoplasia, HSIL is not the most frequent pathology encountered 31 ; it represents the consequence of the study design. Figure 1. Categories of cervical intraepithelial neoplasia - case group Genotypes distribution is presented in Table 3.